Computationally-extracted sections (256 frames) along sagittal planes. The optical sectioning capability and the high resolution are apparent. Despite the highly-scattering structures of the murine embryo, features are well preserved in the image and strongly correlate with the corresponding histology shown in Figure 2.

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Flipbook movie of 256 OCT images of the E10.5 murine embryo acquired transverse to the long-axis of the embryo. Though these sections reveal depth-dependent variations in image quality due to scattering and absorption in the tissue, variations can be minimized by acquiring images from multiple angles, or computationally-sectioning the 3D data set at other angles as shown in Figures 2 and 3.

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Movie III

Dynamic motion of the ventricular and atrial chambers of the E10.5 murine heart. Data for this movie was acquired, processed, and displayed with SD-OCT at a continuous rate of 5 frames/second. Semi-exposure of the E10.5 embryo maintained the embryo-placental continuity, and the embryo remained in situ. This semi-invasive approach yielded more physiologically-relevant data, but resulted in a significantly slower embryonic heart rate. Still frames of this movie are presented in the left column of Figure 6 (A-D).

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Movie IV

Dynamic motion of the E10.5 murine heart. The same heart shown in Movie III was imaged at a different position and angle to reveal the atrial cushion tissue as well as ventricular and atrial chambers. Still frames of this movie are presented in the right column of Figure 6 (I-L).

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